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KMID : 1134820200490010028
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 1 p.28 ~ p.34
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Isolation of Polyphenol Compounds from Ganoderma lucidum and Pancreatic Lipase Inhibitory Activities
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Ryu Eun-Ah
Choi Ji-Hye
Seoung Gi-Un
Chung Shin-Kyo
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Abstract
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In this study, a Ganoderma lucidum methanol extract was fractionated according to the polarity of the solvent, Diaion HP 20 gel column chromatography was used to isolate the polyphenol compounds in the ethyl acetate fraction that showed the highest antioxidant capacity. The anti-obesity effects of each fraction were examined by measuring, the pancreatic lipase inhibitory activities. The polyphenol compounds were identified by HPLC. The antioxidant capacity and pancreatic lipase inhibitory activities in the ethyl acetate fraction were the highest among them (P<0.05). To isolate the polyphenol compounds, the ethyl acetate fraction was obtained with 30%, 60%, and 90% ethanol fractions by Diaion HP 20 gel chromatography. The DPPH (1.93 mM gallic acid equivalent) and ABTS (5.36 mM Trolox equivalent (TE)) radical scavenging activity, FRAP activity (5.92 mM TE), total phenolic content (121.46 mg%), and total flavonoid content (24.91 mg%) in 60% ethanol fraction were the highest (P<0.05). The pancreatic lipase inhibitory activity in the 60% ethanol fraction (1,000 μg/mL) also showed the highest activity as 93.30% (P<0.05). HPLC was used to identify the polyphenol compounds (gallic acid, quercetin, naringenin, kaempferol, and hesperetin) from the G. lucidum methanol extract, ethyl acetate fraction, and 60% ethanol fraction. Among them, the 60% ethanol fraction showed the highest content of total polyphenol compounds. Hesperetin (186.19±5.76 μg/mL) and quercetin (178.60±6.41 μg/mL) were obtained at high levels, followed by naringenin, kaempferol, and gallic acid (P<0.05).
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KEYWORD
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Ganoderma lucidum, solvent fractions, polyphenols, antioxidant, pancreatic lipase inhibitory activity
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